Research: Hoelen Five Herb Formula (Wu Ling San) Has a Protective Effect on Gastric Mucosa


Protective Effects of the Traditional Herbal Formula Oryeongsan Water Extract on

Ethanol-Induced Acute Gastric Mucosal Injury in Rats


Woo-Young Jeon, Mee-Young Lee, In-Sik Shin, Hye-Sun Lim, and Hyeun-Kyoo Shin



This study was performed to evaluate the protective effect and safety of Oryeongsan water extract (OSWE) on ethanol induced acute gastric mucosal injury and an acute toxicity study in rats. Acute gastric lesions were induced via intragastric oral administration of absolute ethanol at a dose of 5 mL/kg. OSWE (100 and 200 mg/kg) was administered to rats 2 h prior to the oral administration of absolute ethanol. The stomach of animal models was opened and gastric mucosal lesions were examined. Gastric mucosal injuries were evaluated by measuring the levels of malondialdehyde (MDA), glutathione (GSH), and the activity of antioxidant enzymes. In the acute toxicity study, no adverse effects of OSWE were observed at doses up to 2000 mg/kg/day. Administration of OSWE reduced the damage by conditioning the gastric mucosa against ethanol-induced acute gastric injury, which included hemorrhage, hyperemia, and loss of epithelial cells. The level of MDA was reduced in OSWE-treated groups compared with the ethanol-induced group. Moreover, the level of GSH and the activity of antioxidant enzymes were significantly increased in the OSWE-treated groups. Our findings suggest that OSWE has a protective effect on the gastric mucosa against ethanol-induced acute gastric injury via the upregulation of antioxidant enzymes.


Copyright © 2012 Woo-Young Jeon et al. This is an open access article distributed

under the Creative Commons Attribution License

1.              Introduction

2.              Material and Methods

1.           Preparation of Oryeongsan

2.           Ethanol-Induced Gastric Injury

3.           Stomach Tissue Histopathology

4.           Biochemical Analysis

5.           Acute Toxicity Study

6.           Statistical Analyses

3.              Results

1.           Acute Toxicity of OSWE

2.           Effect of OSWE on Ethanol-Induced Acute Gastric Injury

3.           Effects of OSWE on MDA Concentration and GSH Levels

4.           Effects of OSWE on the Activity of Antioxidant Enzymes

4.              Discussion

5.              Conclusion

6.              References


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