Inhibition of Mite-Induced Immunoglobulin E Synthesis, Airway Inflammation, and Hyperreactivity by Herbal Medicine STA-1
Tung-Ti Chang, Chieh-Chen Huang, and Ching-Hsiang Hsu
The availability of STA-1 in suppressing allergen-induced immunoglobulin E (IgE) synthesis, airway inflammation and hyperreactivity in a murine model was investigated. The mice were intraperitoneally sensitized with Dermatophagoides pteronyssinus group 5 allergen (Der p 5) and orally treated with 300 mg/kg of STA-1 every other day for 14 days. The Der p 5-specific immunologic responses including changes of specific immunoglobulin G and E, cells in the broncholarvage fluid, and airway hyperreactivity were measured when mice received inhalation challenge with Der p 5 after sensitization for 21 days. By comparing with sham-treated groups, the synthesis of Der p 5-specific IgE was downregulated while the influx of eosinophils and neutrophils in the airway were remarkably reduced. In addition, Der p 5-induced airway hyperreactivity also was significantly eliminated by STA-1 treatment. These results showed that STA-1 could effectively suppress the Der p 5-induced allergic reactions, and the availability of STA-1 for the treatment of allergic asthma was demonstrated in this study.
Copyright © 2006 Chang et al. Published by Immunopharmacology and
Immunotoxicology. All rights reserved.
2. Materials and Methods
3. Sample size
4. Herbal Preparation and Dispensing: The standard herbal formulations (STA-1, STA-2) were prepared and donated by Sun-Ten Pharmaceutical. (Taipei, Taiwan). Both formulations contain LWDHW, made from Radix Rehmanniae Preparata, Cortex Moutan Radicis, Fructus Corni, Poria, Rhizoma Alismatis and Radix Dioscoreae, and MMDT, made from Radix Ophiopogonis, Radix Glycyrrhizae, Radix Panacis Quinquefolii and Tuber Pinellia.
5. Determination of Der p 5-Specific IgG2a and IgE
6. Aerosol Exposure and Analysis of Pulmonary Resistance
7. Bronchoalveolar Larvage and Cell Counting
8. Safety Testing/LD50 Evaluation
9. Statistical Analysis
1. Inhibition of Der p 5-Specific IgE Synthesis
2. Decrease of Airway Hyperreactivity (AHR) in Vivo
3. Suppression of Der p 5-Induced Airway Inflammation
4. Safety of STA-1 and STA-2
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